On-Off Electroretinography (on-off ERG)

The On-Off ERG is a separate protocol used to investigate post-phototransduction or post-receptor dysfunction. This protocol elicits a response from the bipolar and inter-retinal cells. ISCEV indicates that his protocol may further reveal details about: CSNB, melanoma-associated retinopathy, and some forms of autoimmune retinopathy. This protocol may also be useful in X-linked retinoschisis, Batten disease, Duchenne macular dystrophy, spinocerebellar degeneration, quinine toxicity, and other retinal disorders.

This protocol can be done with our flash stimulators: ColorDome, ColorBurst, and ColorFlash. 

Diagnosys has designed a 3-step protocol with step 1 On-response, step 2 Off-response, and step 3 a combined On-Off response. We do so with a ramp down, ramp up, and flicker mechanism, respectively.


Example Tests

A normal patient’s On-response ERG.

On-Response ERG


A normal patient’s Off-response ERG.

Off-Response ERG


A normal On-Off response ERG.

On-Off Response ERG



Sustar, M., Holder, G.E., Kremers, J. et al. ISCEV extended protocol for the photopic On–Off ERG. Doc Ophthalmol 136, 199–206 (2018). https://doi.org/10.1007/s10633-018-9645-y

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